Identification of unknown Bacteria Research Paper

Identification of unidentified Bacteria - Research Paper ExampleWith regards to the aim of the experiment, which is to identify unknown bacteria from mixed stopping point 18 using biochemical and morphological test, the two organisms identified had distinct characteristics which were looked for following laboratory tests. Normally, gram dye is employed to differentiate 2 bacteria groups with different cell wall constituent. The method has the dexterity to differentiate Gram negative and Gram positive bacteria group thus the bacterial cells ar either colo personnel casualty rubor or violet. Gram positive bacteria is shown by Violet staining due to a thick layer of peptidoglycan existing in the cell wall of the bacteria, thus the crystal violet is retained by these cells on a slide. Consequently, Gram negative bacteria is shown by red staining due to a thin layer of peptidoglycan existing in the bacteria cell wall, whence crystal violet is not retained by bacterial cells. (Win n et al,2006)MacConkey Agar is used to distinguish Gram- negative, lactose-fermenting organisms from organisms that are non-fermentative .It is an inhibitory and differential medium that contains Crystal violets, bile salts, inhibitory agent and neutral red. Neutral red acts as a pH indicator. Klebsiella pneumoniae bacteria is known to be lactose fermenters hence unknown A while Salmonella Typhimurium bacteria is not lactose fermentor thus unknown B. The orinithine decarboxylase test is important for Enterobacteriaceae differentiation. (MacFaddin, 2000). MR-VP stock certificate for Methyl Red test consists of peptone, phosphate buffer and glucose.